This might help explain what I have said in an above reply.
Here is the link to this note.
64-bit Windows owners please note that Anime Studio Pro 9 will install both a 32-bit and 64-bit version of Anime Studio in succession on 64-bit machines. You will thus go through 2 installation processes.
64-bit Windows does not support QuickTime as such there are import and export limitations to video/movie and to audio formats.
The only supported movie format for export is .avi. The only supported audio formats for import are .wav, .aif and .aiff. The only supported audio format for export is .wav.
If you require the use of QuickTime, then we recommend using the 32-bit Windows (Universal) version of Anime Studio Pro 9 as it supports QuickTime and video formats such as .mov, .avi, .mp4, .m4v, .mpg, and .mpeg. As well, it supports audio formats such as .wav, .aif, .aiff, .au, .snd, .mp3, .m4a, .mid, .midi, .m4p and .m4b.
64-bit Mac OS X 10.6.x does not support import of QuickTime audio formats (e.g. mp3 and aac), either as audio files or as audio codecs in video files.
To work around this issue, users will have to choose "Open in 32-bit mode" via the application's Get Info window (Select the app in the Finder and either choose Cmd-I or File->Get Info from the main menu). Mac OS X 10.7 and 10.8 do not have this limitation.
When exporting a QuickTime movie with alpha channel on Mac OS, the resulting movie always has a premultiplied alpha channel, regardless of the checkbox that says "Do not premultiply alpha channel."
When importing QuickTime movies with alpha channels, there is a new option in the Image tab of the Layer Settings dialog labeled "Premultiplied alpha". This is not documented in the manual, but allows Anime Studio to support both premultiplied and straight alpha channels. (Previously, Anime Studio only supported straight alpha channels.)